Roboocyte
Two-electrode voltage-clamp recording from Xenopus oocytes has been
widely used to study ion channel function.The advantages of using oocytes
as an expression system to study ion channel function can be attributed
to the availability of sample supply of oocytes, facile handling of the
preparation, rapid injection with cDNA or cRNA, and faithful expression
of exogenous RNA.
Furthermore, ion channel expression can be tuned to levels as desired
by adjusting the amount of cDNA or cRNA injected into oocytes. These characteristics
make the two-electrode voltage-clamp recording from oocytes suitable for
use in an automated recording system.
The Roboocyte (Multi Channel Systems MCS GmbH, Germany) has features of
automated cDNA/cRNA injection and subsequent two-electrode voltage recording
from oocytes in a 96-well plate.
Schnizler, K., Küster, M., Methfessel, C., & Fejtl, M. (2003). The Roboocyte: Automated cDNA/mRNA Injection and Subsequent TEVC Recording on Xenopus Oocytes in 96-Well Microtiter Plates. Receptors & Channels, 9, 41-48.
Automated oocyte injection and recording
- Roboocyte
www.roboocyte.com - Ion channel screening service by the Electrophysiology Group at the NMI
www.nmi.de
